Difficulty

Difficulty can be toggled via the Options menu in Minecraft. Changing this option has a direct impact in the gameplay itself. No setting impacts the quantity of hostile mobs that spawn, including peaceful. There are currently four difficulties in the game.

Realistic mode
Realistic mode is a possibly upcoming game difficulty for Survival in Beta, with several suggestions by Notch.

It will be the hardest difficulty level, requiring you to eat every Minecraft week (140 minutes) and drink every 3 Minecraft days (1 hour).

These statistics imply the game will be much more difficult in this mode.

Hardcore difficulty
Notch said in his blog that he would add a hardcore difficulty level. The rules would be that when you die, the world is deleted and you have to start afresh with a new world. The idea came to Notch from a Minecraft gameplay blog on the PC Gamer site

Tips
If you are having trouble getting started or exploring caverns, the best thing to do is change the difficulty to "Peaceful". You will be able to explore caves or build a base/HQ much more easily. However, this does not give you the full experience, and you will have to turn the difficulty up if you want to get all the items and tools, such as the bow, or bones to make bonemeal. A good recommendation is to turn on Peaceful during the day, and then turn the difficulty back up at night IF you are prepared. This way, you can get both the benefits of the two ways to play.

Another idea is to change it to peaceful whenever you venture into a cave, then turn it back up whenever you leave and go outside or to your house. That way you encounter the enemies above ground at night, but you don't have to worry about a creeper suddenly exploding in the darkness right in front of you or a skeleton shooting at you from someplace you can't see when you go into a cave.

Difficulty does not affect mob spawning in any way.

Trivia

 * Notch made a tweet on October 22nd 2010, stating that "[He's] changing "difficulty" to "realism". Lowest setting = creative. Highest setting = starve".